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Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. Usually, the assay is carried out by determining the enzyme activity with, and without activation by an added coenzyme. The activity can be monitored by measuring changes in concentration of substrates or products during the reaction. Enzyme activity is the rate of enzyme reaction — generally expressed as units of substrate converted (or product formed) per time unit. Enzyme kinetics is the study of the chemical reactions that are catalyzed by enzymes.
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